Application of Raman spectroscopy to study the inactivation process of bacterial microorganisms

Raman spectroscopy (RS) is one of the promising approaches for structural and functional studies various biological objects, including bacterial microorganisms. Both traditional biochemical tests genetic methods which require expensive reagents, consumables are time-consuming used analysis. Spectroscopic positioned as noninvasive, highly sensitive, requiring minimal sample preparation. In this work we investigated possibility using RS method optical sensors based on gold anisotropic nanoparticles. The applicability was demonstrated by studying effect a broad-spectrum cephalosporin antibiotic an extract Viburnum opulus L (VO) Escherichia coli (E. Coli) colonies. were performed Virsa spectrometer (Renishaw). signal amplification carried out two original proposed authors. To create sensors, chemical depositing nanostars APTES-modified quartz glasses physical creating anodizing titanium surfaces. results study showed high sensitivity information content method. inactivation microorganisms shown. Spectral bands E. Coli determined identified before after exposure to VO control. A decrease in intensity spectral modes corresponding amino acids purine metabolites found average spectrum extract. For first time, antimicrobial aqueous fruits scattering. It has been shown that use plant extracts, fruit inactivate vital activity colonies approach search new alternative antibacterial agents. obtained good agreement with already known scientific confirm effectiveness